芦氟沙星与DNA相互作用的研究

论文编号:ZY051    论文字数:11332,页数:25

 摘要：本论文通过荧光光谱、紫外光谱、粘度测定等研究芦氟沙星与DNA的相互作用。分别测定了293K和310K两个不同温度下二者作用的猝灭常数KSV，探讨了其荧光猝灭的机理。在粘度测定中，芦氟沙星与DNA的作用没有引起DNA双螺旋骨架的扭曲变化，而且在紫外吸收光谱中，DNA 的加入并没有对芦氟沙星的吸收峰产生影响，未出现等吸收点；随着芦氟沙星浓度的增加，EB-DNA荧光强度有所减弱，键合饱和时，体系的荧光强度未达50%，故芦氟沙星未发生嵌插作用竞争EB的结合位点，并且在KI的荧光猝灭中，KI对单纯芦氟沙星及与CT-DNA共存的芦氟沙星两种体系的猝灭程度几乎一致，芦氟沙星并未受到DNA碱基对的保护。实验结果表明，DNA对芦氟沙星的荧光猝灭常数为2.89×103L ·mol-1，ΔH=-12.91KJ·mol-1，芦氟沙星与CT-DNA之间不是静电作用，也不是嵌插键合，而是沟槽键合。
 关键词：芦氟沙星；DNA；荧光猝灭；沟槽键合 
 
 Abstract: Fluorescence spectroscopy, UV spectroscopy, viscosity measurement          and other experiments was used to study interaction of rufloxacin with the DNA .Under the temperature of 293K and 310K , the quenching constants  were measured to discusse the mechanism of fluorescence quenching. In the determination of the viscosity experiment, rufloxacin did not distort the DNA backbone. In the UV absorption spectrum,equal absorption point did not appear by the addition of rufloxacin With the increasing rufloxacin concertration,EB-DNA fluorescence intensity was weakened less than 50% suggested that rufloxacin did not compete the EB binding sites.In  the fluorescence quenching of KI experiment, the results were almost same under the simply rufloxacin system and the coexistence of CT-DNA with rufloxacin system.The result suggested that rufloxacin was protected by the DNA bases . In conclusion, rufloxacin interacted with CT-DNA with the quenching constant of 2.89×103L ·mol-1. The interaction is exothermic with a Van’t Hoff enthalply of -12.91KJ·mol-1. Rufloxacin interacted  with CT-DNA in the mode of groove binding .
Key words: Rufloxacin; DNA; Fluorescence quenching; Groove bonding

目   录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
 1.1  概述 1
 1.2 DNA与金属物质的相互作用 2
 1.3 DNA与非金属小分子的相互作用 4
 1.4 药物分子与DNA相互作用的作用本质及影响因素 5
 1.5 芦氟沙星的性质 6
 1.6 研究方法 7
2. 实验部分 8
 2.1 仪器与试剂 8
 2.2 试验方法 8
3. 结果与讨论 10
 3.1 芦氟沙星和DNA相互作用的荧光光谱及热力学研究  10
 3.2 芦氟沙星和DNA相互作用的键和模式 11
4．总结与展望 16
   4.1 实验总结 16
   4.2 展望 16
致谢 18
参考文献 19