加速溶剂萃取仪(ASE100)提取莪术挥发油并采用GC-MS进行分析

论文编号:HG278   论文字数:17632,页数:34

摘  要
 本文采用加速溶剂萃取仪(ASE100)提取莪术挥发油并采用GC-MS进行分析。通过对萃取溶剂类型、提取温度、静态时间以及循环次数对提取效率的影响进行单因素实验,确定各自的最佳条件。实验结果表明,最佳的提取工艺为: 萃取溶剂乙酸乙酯, 萃取温度80℃, 静态时间6min,循环2次。气质联用条件：进样1ul，初始温度60℃，终止温度290℃；质谱扫描范围50-500m/z，扫描间隔0.5s，采用NIST和Wiley谱库对检测物进行在线检索。采用优化后的ASE可快速分离出莪术挥发油中的成分，并结合GC-MS检测。该方法显著优于传统提取和分析方法。
 
 
关键词：加速溶剂萃取;  莪术;  挥发油;  气质联用;

Title: Extraction of Volatile Oil from Rhizoma curcumae-sediment by accelerated solvent extraction and determination by gas chromatography -mass spectrometry
Abstract
In this paper,accelerated solvent extraction (ASE100) method has been used to extract Volatile Oil from Rhizoma curcumae and the main compounds of volatile oil were identified by GC-MS. Single factor experiment has been done to find out the infection of ASE of volatile oil from rhizoma curcumae via solvent,temperature,static period and cycles . Operational parameters were optimized using ethylacetate as the extraction solvent, a temperature of 80℃, a static period of 6 min, two cycles. The GC-MS conditions were as fellows: 1 ul extraction was analysed by GC-MS respectively, the column temperature was maintained at 60 for 2min and increased to 290℃,the scanning range was from m/z 50 to m/z 500(scanning interval:0.5s),and the compounds were identified by searching NIST and Wiley registry of mass spectral data . With the methods of optimized ASE combined with GC-MS,the Volatile Oil from Rhizoma curcumae was extracted and identified rapidly. The method is obviously better than other traditional extraction methods.

Key words： Accelerated solvent extraction;  Rhizoma curcumae；
 Volatile oil；GC-MS;

目  录
第一章  文献综述 1
1 .1加速溶剂萃取技术 1
1.1.1 ASE的原理 1
1. 在高温下萃取 1
2. 在加压下萃取 1
1.1.2 加速溶剂萃取仪 2
1.1.3 ASE与其它新兴提取技术的比较 3
1.1.4 ASE的应用 6
1.2 莪术 9
1.2.1 莪术的分类 9
1.蓬莪术 9
2.广西莪术 9
3.温莪术 10
1.2.2 莪术的采收和储藏 10
1.2.3莪术的中药化学成分 11
1.2.4 莪术有效成分的提取方法 12
1.2.4.4 多糖类成分的提取 13
1.2.5  药理研究 13
1.2.6 莪术的临床应用 16
1.3 ASE法萃取中药挥发油的研究展望 18
第二章 实验部分 19
2.1 实验药品及仪器 19
2.1.1实验药品 19
2.1.2仪器 19
2.2 实验方法 19
2.2.1 ASE萃取的条件 19
2.2.2 萃取液测定的GC-MS条件 19
第三章  结果与讨论 21
3. 1 　ASE萃取条件的选择 21
3.1.1 提取溶剂的选择 21
3.1.2 提取温度的选择 21
3.1.3提取时间的选择 22
3.1.4 循环次数的选择 22
3.2 GC-MS分析 23
3.3 ASE法萃取蒙药暖宫七味丸的挥发油成分 25
第四章  结论 26
致  谢 27
参 考 文 献 28