积雪草苷的高效分离制备方法研究

论文编号:SW194  论文字数:10740,页数:26

摘要  采用溶析结晶法从积雪草总苷中高效制备高纯度积雪草苷。通过考察不同积雪草总苷于各种溶析剂下的溶解度，以及考察了不同溶剂—溶析剂体系下从积雪草总苷分离制备积雪草苷的规律。得到了98.1%以上纯度的积雪草苷。该方法工艺简单，大大地降低了生产成本以及生产过程中的能源消耗，减少了污染排放。

关键词  积雪草；积雪草苷；溶析结晶

Abstract   A crystallization method is applied to separate asiaticoside from  total glucosides of Centella asiatica. Through the study of different solubility of total glucosides of Centella asiatica under a variety of melting-out agent and the resolution in different solvents - melting-out agent system were observed. More than 98.1% purity asiaticoside was obtained. The method is simple process, lower the prime cost and energy consumption, reducing pollution emissions.

Keywords   Centella asiatica; Asiaticoside; Crystallization

目  录

中文摘要……………………………………………………………………………I
英文摘要……………………………………………………………………………II
目录…………………………………………………………………………………III
1.绪论………………………………………………………………………………1
2.实验部分…………………………………………………………………………6
 2.1实验材料………………………………………………………………………6
 2.2实验试剂与仪器设备…………………………………………………………6
 2.2.1试剂………………………………………………………………………6
 2.2.2仪器设备…………………………………………………………………6
 2.3色谱条件………………………………………………………………………6
 2.4实验方法………………………………………………………………………7
 2.4.1  积雪草总苷溶解度考察………………………………………………7
 2.4.2  溶析剂选择……………………………………………………………7
 2.4.3  考察溶析结晶分离规律………………………………………………8
 2.4.4 98%以上纯度的积雪草苷制备…………………………………………9
3, 结果与讨论………………………………………………………………………10
 3.1  溶剂筛选……………………………………………………………………10
 3.2  溶析剂筛选…………………………………………………………………10
 3.3  溶析剂种类对分离效果的影响……………………………………………12
 3.4  溶剂对分离效果的影响……………………………………………………15
 3.5  积雪草总苷浓度对分离效果的影响………………………………………17
 3.6  高纯度积雪草苷制备………………………………………………………19
4. 总结与展望………………………………………………………………………19
 致谢…………………………………………………………………………………21
 参考文献……………………………………………………………………………22