酸杆菌的固定化及其发酵特性的研究

论文编号:SW061  论文字数:14982,页数:33

摘  要：固定化细胞技术是20世纪60年代发展起来的一门新兴生物技术，是近十余年来生物工程研究的重点领域之一。现在，固定化酶及固定化细胞技术在酶工程中占有十分重要的地位。随着细胞固定化技术的日益成熟，固定化细胞在工业上的应用越来越广泛。本课题利用海藻酸钠包埋法对醋酸杆菌1.01进行了固定化，确定了细胞的固定化条件。以不同菌体浓度，不同颗粒直径制备固定化细胞，测定了固定化细胞发酵过程中的产酸特性、菌体生长特性、底物消耗特性等，并与游离态醋酸杆菌发酵进行了比较，反映了固定化醋酸杆菌的发酵特点，并为固定化醋酸杆菌发酵动力学的研究提供了实验数据。

关键词：固定化细胞；醋酸杆菌；发酵特性

Immobilization of Acetobacteria and Fermentation Characteristics Research

Abstract：The immobilized cell technology is becoming more and more important since its development in 20th century 60''s. Now, it has been one of the key domains of bioengineering research and gained widespread industry applications. In our work, sodium alginate was used as the carrier to immobilize Acetobacteria 1.01 by embedding method. The optimal embedding conditions were studied. Immobilized particles with different cell density and different diameter were obtained and used for fermentation. During fermentation process, cell growth, substrate consumption and vinegar production were determined and compared with that of free cell fermentation .This work reveals the fermentation characteristics of immobilized Acetobacteria 1.01 and provides data for fermentation kinetic research.

Key words：Immobilization cell; Acetobacteria; Fermentation characteristic

目  录
中文摘要 I
英文摘要 II
目录 III
1 绪论 1
1.1 前言 1
1.2 固定化细胞的概论 1
1.3  海藻酸钠包埋法在细胞固定化中的应用及发展前景 3
2 实验部分 6
2.1 实验材料 6
2.2 实验方法 8
2.3 实验流程 13
2.4 培养方法 13
2.5 固定化条件的优化实验 14
2.6 固定化醋酸杆菌发酵特性的研究 16
3 结果与讨论 18
3.1 固定化条件的优化 18
3.2 固定化醋酸杆菌的发酵特性研究 19
4 总结与展望 25
致谢 27
参考文献 28