响应面法优化裙带菜粗多糖提取工艺的研究

论文编号:SP117  论文字数:9601,页数:23

摘要：多糖具用抗病毒、抗凝血等多种生理活性，本文对裙带菜中粗多糖的提取条件进行了研究。试验采用甲醇对新鲜裙带菜进行预处理，除绝大部分的色素，用热水浸提，采用不同浓度乙醇进行分级沉淀,蒽酮硫酸法检测多糖主要存在于当乙醇浓度为60％的沉淀中。在单因子试验基础上，选取裙带菜粗多糖提取时间、提取温度和料液比3个因素进行中心组合设计，利用响应面分析法(RSA)对粗多糖的提取工艺进行优化。依据回归分析确定工艺条件的影响因子，在分析各个因素的显著性和交互作用后，从而得出最佳水浸提工艺条件：提取温度75.8℃、水料比6.3∶1、提取时间2.2h、提取2次，裙带菜粗多糖的糖含量为7.73％。
关键词：裙带菜；提取；单因子试验；响应面法
 
Abstract：The polysaccharide has a variety of biological activities, such as anticoagulant activities and antiviral activities. In this paper extraction conditions of Undaria pinnatifida polysaccharides were studied. The polysaccharide was extracted from fresh Undaria pinnatifida which was pretreated with methanol to get rid of most pigment by hot water, then ethanol was used to make polysaccharide precipitated. Anthrone-sulfuric acid was used to determine the content of polysaccharide and the result showed that most polysaccharide was found in the deposition when the consistence of ethanol was 60%. On the base of the single factor experiment and a three-level three-factor Box-Behnken design, the effects of different temperature, extract time and the ratio of solid to solution on the yield of the polysaccharide were investigated. The extraction technique parameters were optimized with Response Surface Methodology and the optimum conditions of extraction were as follow: the temperature 75.8℃, the extraction time 2.2h, extraction twice, ratio of water to solution 6.3∶1, In this condition, extraction rate of Undaria pinnatifida polysaccharide was 7.73%.
Keywords：Undaria pinnatifida; Extraction; Single-factor test; Response surface methodology

目录
中文摘要 I
英文摘要 II
目录 III
1  绪论 1
1.1 裙带菜简介 1
1.2 多糖研究的现状 2
1.3 BOX-BEHNKEN试验设计概述 4
1.4 研究的目的和意义 4
2  实验部分 6
2.1 材料 6
2.1.1 原料 6
2.1.2 试剂和设备 6
2.2 实验方法 7
2.2.1 工艺流程 7
2.2.2 葡萄糖含量标准曲线的绘制 7
2.2.3 热水浸提工艺条件优化 8
3  结果与讨论 10
3.1 各个乙醇浓度沉淀之间的比较 10
3.2 单因子试验影响研究 10
3.2.1 提取温度对多糖的糖含量影响 10
3.2.2 提取时间对粗多糖的糖含量的影响 11
3.2.3 水料比对粗多糖的糖含量影响 11
3.2.4 pH对粗多糖的糖含量影响 12
3.3 响应面分析方案及结果 13
4 总结与展望 17
致  谢 18
参考文献 19