猪源双歧杆菌发酵工艺的研究

论文编号:SP035  论文字数:13304,页数:31

摘  要

 双歧杆菌是人体内存在的一种生理性细菌，是人体有益菌中最值得重视和研究的一种，它与人体的健康密不可分，可以说是大自然赐予人类的健康法宝。双歧杆菌的研究和生产具有很好的发展前景。
 双歧杆菌一般能从动物身上提取，猪的胃上皮细胞黏膜附有有大量双歧杆菌。从猪的粪便中分离出双歧杆菌，并将其扩大生产。合适的生长条件对双歧杆菌的生长速度、菌体形态、活菌数、代谢产物有良性的影响。因此通过研究，获得双歧杆菌生产的最优工艺方案有非常重要的意义。

关键词：双歧杆菌  发酵工艺 
 
 
 THE STUDIES OF PIGGED-SOURSE BIFODOBACTERIUM FERMENTATION PROCESS
ABSTRACT
 
 Bifodobacterium is a sort of physiological bacteria inside human body, and is also one of the probiotics which deserves to be valued and researched on. It is inseparably connected with human health and could be described as a treasure of heath given to humanity by the nature. Therefore, the research and production of bifodobacterium could prove to be promising.
 Bifodobacterium is partly extracted from an animal, like a pig, who has a huge amount of epith cells of gastric mucosa. Thus bifodobacterium could be isolated from the excrement of pigs and the cultures could be expanded. A proper condition of culture has a positive effect on the growth speed, morphological characteristics, number of vial bacteria and metabolites of bifodobacterium. Therefore this research is crucially important to acquire an optimal technology scheme of the culture of bifodobacterium.

Key Words: bifodobacterium  fermentation process
 
 
  
 
 目  录
 
摘  要 I
 ABSTRACT II
1引言 1
1.1 研究背景 1
 1.1.1 研究单位简介..........................................1
1.2 双歧杆菌的作用 2
 1.2.1 改善人体肠道功能......................................2
 1.2.2 恢复人体菌群平衡，形成抗菌生物屏障，维护人体健康......2
 1.2.3 控制人体内毒素的水平，保护肝脏病增强肝脏的解毒、排毒功能
 ............................................................2
 1.2.4 降血脂作用，防止动脉硬化个高血压.......................2
 1.2.5 免疫调节作用...........................................3
 1.2.6 营养作用...............................................3
 1.2.7 提高SOD酶活力，消除人体自由基.........................3
 1.2.8 抗肿瘤、预防癌症.......................................4
 1.3 研究意义.................................................4
 1.3.1双歧杆菌开发现状........................................4
 1.3.1.1 双歧杆菌因子的开发与应用 ............................4
 1.3.1.2 双歧杆菌营养液 ......................................4
 1.3.1.3 新型酸奶的开发与应用 ................................5
 1.3.1.4 双歧杆菌药品的开发...................................5
 1.3.2 双歧杆菌生物工程展望...................................5
 2  材料..................................................7
 2.1样品来源..................................................7
 2.2主要实验试剂..............................................7
 2.3主要仪器与设备............................................7
 3 双歧杆菌的分离和鉴定..................................8
 3.1实验方法..................................................8
 3.1.1 分离纯化方法...........................................8
 3.1.2 厌氧培养...............................................8
 3.1.3 仔猪肠道双歧杆菌的初步鉴定.............................8
3.2 本章小结............................................9
4仔猪肠道双歧杆菌的生长特性研究 10
4.1 操作方法 10
 4.1.1 试验方法 10
4.1.2 检测方法 10
 4.2 培养温度 10
4.3 起始pH.............................................11
 4.4 培养基的选定............................................12
 4.4.1 碳源..................................................12
 4.4.2 氮源..................................................12
 4.4.3 生长因子..............................................12
 4.5测定双歧杆菌的生长曲线............................13
 4.6 本章小结.............................................15
5  双歧杆菌发酵工艺条件的优化研究........................16
 5.1 双歧杆菌制剂的生产工艺流程..............................16
 5.2 发酵方案的确定..........................................16
 5.3 厌氧发酵培养基组成及发酵条件的确定......................16
 5.3.1 厌氧发酵的培养基组成..................................16
 5.3.2 发酵控制条件..........................................16
 5.4 发酵液活菌数的检测......................................16
 5.5 10L发酵罐扩大培养试验..................................17
 5.6 100L发酵罐扩大培养试验................................18
 5.7 本章小结...............................................18
 6  结论与展望...............................................19
 6.1 结论....................................................19
6.2 展望...............................................19
参考文献 20
附录I 培养基的配置 21
 附录II  双歧杆菌的糖代谢途径...........................22
谢辞 24