紫莳药中花青素的提取

论文编号:SP066  论文字数:10296,页数:23

摘要：花青素拥有高效的抗衰老、抗心血管疾病、抗癌功能，还具有抗辐射、抗疲劳，改善记忆力等作用，尤其在清除体内有害自由基方面显示出了无比优越的生物活性和安全性。但人体自身无法合成花青素，需从外界摄入，因此研究如何高效提取高纯度花青素是有意义和应用前景的。
 本文对采用溶剂萃取法萃取紫莳药中的花青素进行了研究，着重考察了浸提剂浓度、萃取时间、萃取温度及料液比等因素对花青素得率的影响。在单因素实验基础上进行正交实验，通过直观分析和方差分析确立了萃取花青素工艺的初步优化工艺条件：提取剂用体积分数为60%的乙醇溶液,料液比1:3,浸提温度60℃,浸提时间13h。在该优化条件下提取，花青素的得率为3.90%。

关键词：紫莳药；花青素；溶剂萃取；正交设计

Abstract：Anthocyanins have effective anti-aging, anti-cardiovascular, anti-cancer functions, and also play a role in anti-radiation, anti-fatigue and improving memory, especially in the respect of clearing harmful free radical of the body. They have showed extremely superior bioactivity and safety. However, our bodies can’t produce any anthocyanins, we should only take them from food or other supplements. It has been reported that extraction and preparation of high purity anthocyanins has wide application prospects.
   This work studied  the extraction of anthocyanins from dioscorea batata decne. The effects of solvent concentration, ratio of sample to solvent, extraction temperature and extraction time on the yield of anthocyanins were studied. Based on the data of single-factor experiment, the optimum conditions were evaluated further applying the orthogonal testing method with intuitive analysis and variance analysis. The preliminary majorized extraction conditions are as follows: extraction solvent of 60% (volume fraction) alcohol solution, ratio of sample to solvent 1:12 (g/mL), extraction temperature 60 ℃, extraction time 13 h. Under these conditions, the yield of anthocyanins is 3.90%.

Keywords: Dioscorea Batata Decne；Anthocyanins；Solvent Exhaustion；Orthogonal Design
目  录
中文摘要 Ⅰ
英文摘要 Ⅱ
目录 III
1.绪论 1
1.1研究的背景 1
1.2研究的意义 2
1.3花青素的简介 3
1.4实验方案 6
2.实验部分 7
2.1实验原理 7
2.2实验试剂和材料 7
2.3实验仪器和设备 7
2.4实验步骤 8
3.结果与讨论 10
 3.1标准曲线 10
3.2单因素实验结果与讨论 10
3.3正交实验结果与讨论 13
3.4实验结论 15
4.总结与展望 16
致谢 17
参考文献 18