【编者按】：网学网药学为您提供茶皂素提取工艺的研究参考，解决您在茶皂素提取工艺的研究学习中工作中的难题，参考学习。
论文编号:ZY093 论文字数:7037,页数:17
摘 要：油茶是山茶科植物属植物油,我国是世界上油茶籽产量最大、品种最多的国家。油茶籽饼中含有丰富的三萜皂甙。油茶皂甙是一种良好的表面活性剂，有较好的洗涤效果，耐高浓度电解质能力也较好，乳化、起泡、抗静电能力都比较强，可用于碳酸饮料的发泡剂、高级洗涤剂、灭火剂、乳化剂；油茶皂甙还有良好的药用价值，具有溶血、消炎、祛痰、抗真菌、抗突变的作用。本论文对从茶粉末中提取茶皂素的生产工艺进行研究，对影响茶皂素收率的因素进行了分析讨论，优化纯化提取工艺。并用高效液相色谱仪对茶皂素的含量进行定量分析。得含量为94%。
关键词：油茶籽饼；茶皂素 ；提取；高效液相色谱
Abstract：Camellia is a vegetable oil, which is the world''''s largest varieties camellia oil in China. Triterpenoid Saponins is rich in camellia oil cake. Camellia Saponin is a good surfactant, a better washing effect of high concentrations of electrolyte resistance ability to better emulsification, foaming, anti-static capacity than the stronger of carbonated drinks can be used to foam, senior scullery agents, fire extinguishing agent, emulsifier，the medicinal value of Saponin are good with hemolytic, anti-inflammatory, expectorant, anti-fungal, anti-mutation effect. In this paper, study the production process of Tea Saponin extracting from the tea saponin, and discuss the impact of yield factors and optimize the extraction and purification. The quantitative analysis of Tea Saporin by reverse phase HPLC is taken．The results shown content is 94%.
Keywords：Camellia oil；Tea Saponin；Extract；HPLC
目录
中文摘要 ..........................................................I
英文摘要.......................................................... II
目录 ........................................................... III
1. 绪论 ...........................................................1
1.1 引言 .......................................................1
1.2 茶皂素的主要提取方法 ........................................2
1.2.1 正丁醇萃取法 ........................................2
1.2.2 重结晶法 ............................................2
1.2.3 吸附法 ..............................................2
1.2.4 聚酰胺柱层析法 ......................................3
1.2.5 硅胶柱层析 ..........................................3
1.2.6 离子交换树脂法 ......................................3
1.2.7 絮凝剂法.............................................3
1.2.8 超滤膜法.............................................3
1.2.9 大孔树脂法...........................................4
1.3 皂甙的主要结构鉴定方法.......................................4
2. 实验部分.........................................................5
2.1 试剂与仪器...................................................5
2.1.1 材料..................................................5
2.1.2 主要试剂...............................................5
2.1.3 主要仪器设备...........................................5
2.2 实验步骤....................................................... 6
2.2.1 大孔吸附树脂的预处理..................................6
2.2.2 大孔吸附树脂的再生................................... 6
2.2.3 大孔吸附树脂吸附..................................... 6
2.2.4 高效液相色谱分析..................................... 6
3. 结果与讨论.......................................................7
3.1 影响茶皂素收率的因素分析.....................................7
3.1.1 乙醇浓度对茶皂素的收率的影响..........................7
3.1.2 吸附次数的影响........................................7
3.1.3 过柱吸附速度的影响....................................8
3.1.4 吸附过程最佳工艺参数的确定............................9
3.2 茶皂素含量分析.............................................. 9
3.3 讨论........................................................ 9
4. 总结与展望..................................................... 10
致谢...............................................................12
参考文献...........................................................13
