响应面法优化提取蒲公英黄酮工艺研究

论文编号:ZY053    论文字数:9736,页数:21

摘要：本研究采用乙醇提取法从蒲公英中提取黄酮，考查了乙醇体积分数、料液比、提取温度和提取时间等因素对蒲公英黄酮提取效果的影响。为确定蒲公英中黄酮类成分乙醇提取的最佳工艺条件，以黄酮得率为指标，在单因素试验基础上采用响应面法对主要工艺参数进行优化并得到回归模型。方差分析结果表明：回归模型较好地反映了蒲公英黄酮得率与提取时间、提取温度、乙醇体积分数和料液比的关系；最佳工艺条件为提取时间52min、提取温度65℃、乙醇体积分数58%、料液比1∶20g/ml。此工艺条件下提取蒲公英黄酮得率为8.16%（以芦丁计），回归模型的预测值与实际值的相对误差为1.3%。该优化工艺提高了黄酮的提取率，取得了较好的成效。
关键词：蒲公英；黄酮；提取工艺；响应面法

Abstract: The ethanol extraction of flavonoid from Taraxacum mongolicum were carried out. The effects of concentration of ethanol, ratio of solvent to raw material， temperature and extracting time on extraction effect of flavonoid from Taraxacum mongolicum were discussed. With the aim of optimization of the ethanol process for extracting flavonoid from Taraxacum mongolicum, a central composite design(CCD) experiment was conducted and the response surface methodology(RSM) based on single factor was employed to investigate the influence of four main factors on the yield of flavonoid, and to establish a regression model. Regression coefficient and variance analysis show the regression model fits the actual process well. The obtained optimum extraction parameters are extracting time 52min, temperature 65℃, concentration of ethanol 58%, and ratio of solvent to raw material 1:20. The flavonoid yield with the optimized procedure is 8.16%(as rutin). The experimental values agree with those predicted from the regression model within a relative error of 1.3%, Applying this extracting technology, the flavonoid content is increased, the results showed that this process is reasonable and practicable.
Key words: Taraxacummongolicum; flavonoids; extraction technology; response surface methodology

目  录
中文摘要 I
英文摘要 II
目录 III
1．绪论 1
   1.1蒲公英介绍以及黄酮类化合物生物活性介绍 1
   1.2响应面分析法的介绍 3
2．实验部分 5
 2.1材料与仪器 5
 2.2实验方法 5
3．结果与讨论 7
 3.1提取时间对蒲公英黄酮提取率的影响 7
 3.2提取温度对蒲公英黄酮提取率的影响 7
 3.3乙醇浓度对蒲公英黄酮提取率的影响 8
 3.4料液比对蒲公英黄酮提取率的影响 9
 3.5黄酮得率回归模拟建立及显著性检验 9
 3.6蒲公英黄酮提取的响应面分析与优化 12
 3.7蒲公英黄酮最佳提取条件的确定和试验验证 14
4．总结与展望 16
致谢 17
参考文献 18