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采用生物指示剂对臭氧消毒效果探讨摘 要臭氧消毒灭菌技术以其消毒效果好、使用方便、对作业人员和作业场所没有危害，现在为制药行业广泛采用，主要用于在十万级条件下臭氧对环境的消毒、灭菌。在微生物挑战性实验中，传统的检验臭氧消毒效果是用枯草芽孢杆菌来检测，但实验证明枯草芽孢杆菌作为检测的指示剂存在着较多的缺点，其杀菌率达不到微生物挑战性实验的标准要求。为提高检测的灵敏度，本实验分别考察以金黄色葡萄球菌和枯草芽孢杆菌作为微生物指示剂，在不同的臭氧消毒时间、臭氧浓度、温度和湿度等条件下，环境中的菌体残留量来检测杀菌效果。实验数据表明金黄色葡萄球菌在消毒时间为2小时，臭氧浓度在10-15ppm之间，温度在18-26℃，湿度在60%时，其检测效果比枯草芽孢杆菌灵敏，因此可选用金黄色葡萄球菌对臭氧消毒后的环境进行检测。关键词： 生物指示剂 臭氧 消毒The use of biological indicator of ozone disinfection AbstractOzone disinfection and sterilization technology for its effective, easy to use, for operators and workplaces no danger, now widely used for the pharmaceutical industry, mainly for the one hundred thousand grade on the environment under the conditions of ozone disinfection, sterilization. In the microbial challenge experiments, the traditional test of ozone disinfection is used to detect Bacillus subtilis, but the results show detection of Bacillus subtilis as an indicator of the existence of more defects, the bactericidal rate was less than a microbial challenge experiments standards. To improve detection sensitivity, were investigated in this experiment in order to Staphylococcus aureus and Bacillus subtilis as an indicator micro-organisms, at different time of the ozone disinfection, ozone concentration, temperature and humidity conditions, the environment to detect the amount of biomass residues bactericidal effect. Experimental data show that the disinfection of Staphylococcus aureus in time is 2 hours, the ozone concentration in the 10-15ppm between the temperature of 18-26 ℃, humidity between 60 ~ 65%, its detection sensitivity better than Bacillus subtilis, it Staphylococcus aureus can be selected after ozone disinfection of environmental testing. Key words: aureus subtilis; ozone; microbial challenge test
目录摘 要1Abstract2第一章 绪论51.1臭氧的性质51.2臭氧消毒原理51.3臭氧技术在制药工业上应用范围51.4生物指示剂菌种71.5本课题的研究目的和意义8第二章 实验材料与方法92.1 实验材料与仪器92.1.1 主要实验仪器92.1.2 主要实验试剂92.2 实验方法92.2.1实验菌株92.2.2实验试剂102.3 实验方法102.3.1实验步骤102.3.2 菌落的计数112.3.3 杀菌率11第三章 结果与讨论123.1 消毒时间对两种不同菌种的影响123.1.1 消毒时间对金黄色葡萄球菌的影响123.1.2 消毒时间对枯草芽孢杆菌的影响123.2 臭氧浓度对两种不同菌种的影响133.2.1 臭氧浓度对金黄色葡萄球菌生长的影响143.2.2 臭氧浓度对枯草芽孢杆菌生长的影响143.3 消毒时温度对两种不同菌种的影响163.3.1温度对金黄色葡萄球菌的影响163.3.2温度对枯草芽孢杆菌的影响163.4 消毒时湿度对两种不同菌种的影响173.4.1湿度对金黄色葡萄球菌的影响173.4.2湿度对枯草芽孢杆菌的影响18第四章 对检验杀菌效果的方法达标与否的验证204.1金黄色葡萄球菌检测条件204.2对金黄色葡萄球菌做微生物指示剂验证204.2.1验证方法204.2.2浮游菌检测204.2.3金黄色葡萄球菌检测204.3杀菌率20参考文献22致谢23
采用生物指示剂对臭氧消毒效果探讨......
