均匀试验及其在药物研制优化设计中的应用

论文编号:HG120  论文字数:18659,页数:38

摘要：基于均匀设计具有实验次数少，因素的水平也可以适当调整的特点，故它在寻找最佳实验条件、最佳配比等方面是选择优化条件的有力工具。均匀试验设计在药学研究上的应用十分广泛，主要应用于药物合成、药物分析、药剂组方和药理实验条件优化等方面上。本文对试验设计的方法进行了介绍，探讨了均匀设计的特点，均匀设计表的构造及其在药物研制中的应用。
 目的是优化四君子汤提取的工艺条件。采用均匀设计法，以人参总皂甙和总多糖为指标，探讨了四君子汤的最佳提取工艺条件。优化结果表明以己醇浓度10﹪，溶剂用量为药材量的13倍，浸泡时间为14h，回流时间为2h为佳。得出的结论是均匀试验设计方法成功地实施了四君子汤提取方法的优选，简单易行, 符合生产实际。
关键词：均匀设计；正交设计；试验方案；药物研制 ；优化设计

Uniform design and its application in studying and optimizing new drugs
Abstract: Uniform design has the characteristics of fewer experimental times, and the level of factors which can be appropriately adjusted, so it is a powerful tool for choosing optimal conditions, especially in looking for the best experimental conditions, the best chosen ratio and so on. Uniform Design had a wide range of applications in pharmaceutical research, mainly used in areas such as drug synthesis, drug analysis, the Pharmacy Group and the Optimization pharmacological experiments. The article introduces the method of experimental design and discusses the feature and application of uniform design on studying new drugs, as well as the designing of uniform design form.
 The objective is to optimize the extracting technology of Si junzi Decoction. The optimal extracting technology of Si junzi Decoction was selected using Ginseng saponins and total polysaccharides as an index by a uniform design method. The results indicated that the optimal conditions were as follows：the concentration of alcohol was 10﹪(v／v)，the solvent quantity was 13 times as much as the drug quantity，the soak time was 14 hours and the reflux time was 2 hours. We can obtain the conclusion that the optimized procedure of uniform design succeeded in the optimal extracting technology of Si junzi Decoction. It is simple and easy to do, and suitable for practical production.
Key word: uniform design; orthogonal design; experimental scheme; studying drugs；optimum design

目  录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
 1.1 试验设计概述 1
 1.2 常用试验设计方法 5
 1.3 试验结果的数据处理方法 12
 1.4 试验设计在科学研究中的地位与意义 13
2. 均匀试验设计 15
 2.1 均匀试验设计的基本思想 15
 2.2 均匀试验设计的特点 15
 2.3 均匀试验设计表的构造 16
 2.4 均匀设计表的特点 20
 2.5 均匀设计表的使用 21
 2.6 均匀设计与正交设计的比较 21
 2.7 均匀设计的步骤 22
 2.8 均匀设计的数据处理 22
3. 均匀设计的实际应用 23
 3.1 药物研制的试验条件的确定  24
 3.2 设计试验方案 24
 3.3 数据处理 25
 4.结果与讨论 28
5．总结与展望 29
致谢 30
参考文献 31
附录 33