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论文编号:HG057 论文字数:12913,页数:25
摘 要：本论文以贻贝为原料，它是重要的世界性养殖品种，以紫贻贝产量占有最大，并以新鲜贻贝为主要销售形式，经济效益低。贻贝不但作为传统的滋补食品，且具有较高的营养价值和多种药理功效。文献报道，贻贝及其提取物具有抗氧化、免疫调节、抗肿瘤、降血脂等功能活性。其中，多糖是紫贻贝中的主要活性成分之一，目前对其研究主要集中在紫贻贝粗多糖上，而对紫贻贝多糖组分的提取、分离纯化及结构功能研究报道较少。因此，开发利用紫贻贝多糖具有理论价值和实际意义。本课题以嵊泗枸杞所产紫贻贝为试验材料，研究了紫贻贝多糖的提取、脱蛋白、分离纯化、结构鉴定及其免疫抗肿瘤活性。为了鉴定世界食品产品中受到很高评价的海洋生物的抗氧化性，采用酶解方法制备具有显著抗氧化作用的活性肽。主要研究结果如下：在DPPH自由基体系、还原力体系、羟基自由基体系及超氧自由基体系中，随着样品浓度的增加，贻贝酶解物的抗氧化活性增强。
关键词：贻贝；DPPH· ；抗氧化；还原力；超氧；
Study on antioxidant activity of mussel enzymatic peptides
Abstract: This article uses mussel as materials, it is an important aquaculture species worldwide, the ratio of Mytilus edulis (ME) is the largest. But people could not make more profits if only mussel was sold directly. Mussel not only is a traditional tonic food, but also has a high nutritional value and variety of pharmacological effects. According to related materials, both mussel and its extracts have variety of functional effects, such as anti-oxidant, immune regulation, anti-tumor, antihypertensive effect and so on. Polysaccharides are one of the major chemical ingredients of ME. And now more researches were focused on the crude polysaccharides from ME, however, the studies about the extractions, purifications and structures and functional researched systemly have not yet reported. Therefore, ME were obtained from an island named wolfberry in Zhoushan and then employed to extract polysaccharides. Extraction, isolation, structure identification and bioactivity of polysaccharides from ME were further studied. The major results obtained in this study were as follows: In DPPH radical and restore the power system and hydroxyl radical and super oxygen radical system, with sample the concentration of whom we shall solve the enzyme antioxidant activity enhance.
Keywords：Mussel; DPPH·; antioxidat; reducing power; super oxygen;
目 录
中文摘要 I
英文摘要 II
目录 IV
1. 绪论 1
1.1 自由基代谢理论 1
1.2 自由基对生物机体的损伤及机制 1
1.3 自由基的清除及防御措施 3
1.4 抗氧化剂概述 4
1.5 体外评价抗氧化的方法 5
1.6 实验背景 6
2. 实验部分 6
2.1 实验材料 6
2.2 实验试剂 7
2.3 实验仪器 7
2.4 实验方法 7
3 结果与讨论 10
3.1 基本成分分析 10
3.2 不同蛋白酶对贻贝粗蛋白的水解效果的比较 10
3.3 DPPH自由基体系 11
3.4 还原力的测定 12
3.5 羟基自由基体系 13
3.6 超氧自由基体系 14
4 结 论 15
4.1 实验结论 15
4.2 实验条件讨论 16
致 谢 17
参考文献 18
