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论文编号:HG075 论文字数:11161,页数:24
摘 要：本文采用了三种不同的提取方法:回流提取法、乙醇浸提法、超声提取法对一枝黄花的叶子进行提取。结果显示: 乙醇浸提法提取液杀虫活性最好，甲醇超声提取法所得提取液次之，水回流提取液最弱。根据上面的比较结果，用紫外分光光度计检测三种不同提取方法下提取的提取液含量，用高效液相色谱分析乙醇浸提法的提取液成分，得出是黄酮类物质、皂苷、酚类物质、精油起了关键作用，这些物质均具有药理活性，具有抗炎、抗菌、利尿、抗肿瘤活性等作用，根据菜虫的死亡率进行讨论和总结。随着外来入侵植物加拿大一枝黄花在我国迅速的泛滥危害,有关该植物研究逐渐增多，但对一枝黄花属植物的化学成分及其生物活性尚缺乏系统研究，根据一枝黄花的杀虫活性分析它的化学成分急需进行。
关键词：加拿大一枝黄花；杀虫活性；黄酮类；紫外分光光度计；高效液相色谱
Solidago insecticidal activity component analysis
Abstract: This paper based on the three different extraction methods: ethanol refluxing extraction, ultrasonic extraction, extraction of a yellow leaves were extracted. Results showed that ethanol extraction extract insecticidal activity is best, methanol ultrasonic extraction, water extract from the backflow extract. According to the comparison results, use on uv spectrophotometer detection under three different extraction methods of extraction and extract content by HPLC analysis of ultrasonic extraction of methanol extraction liquid, that is the flavonoids, saponins, phenolics, oil plays a key role, these substances are pharmacological activities have anti-inflammatory, antibacterial, diuresis, antitumor activity, according to the food are discussed and summarized. Death As invasive plants solidago canadensis of flood hazards in China quickly on the plant, increase gradually, but research on a flower of chemical composition and the biological activity, lack of system according to a yellow insecticidal activities of chemical composition analysis, it urgently.
Keywords: Solidago Canadensis; Insecticidal activity; flavonoids ;UV ; HPLC
目 录
中文摘要 ………………………………………………………………………… Ⅰ
英文摘要 ………………………………………………………………………… Ⅱ
目录 ……………………………………………………………………………… Ⅲ
1.绪论 …………………………………………………………………………… 1
1.1一枝黄花的主要化学成分 ………………………………………………… 1
1.2 一枝黄花的红外光谱图………………………………………………………2
1.3 加拿大一枝黄花精油的化学组成……………………………………………3
1.4 一枝黄花的生物性能…………………………………………………………5
1.5 一枝黄花的应用现状…………………………………………………………7
2．实验内容 …………………………………………………………………………8
2.1研究的主要内容………………………………………………………………8
2.2实验材料与仪器………………………………………………………………8
2.3实验步骤………………………………………………………………………9
3.检测方法……………………………………………………………………………9
3.1紫外可见风光光度计……………………………………………………………9
3.2高效液相色谱仪…………………………………………………………………10
3.3标准曲线的绘制…………………………………………………………………10
3.4样品测定…………………………………………………………………………11
4.结果与讨论…………………………………………………………………………12
4.1紫外分光光度计的测定结果……………………………………………………13
4.2高效液相色谱测定结果…………………………………………………………16
5.前景展望……………………………………………………………………………17
致谢……………………………………………………………………………………18
参考文献………………………………………………………………………………19
