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论文编号:HG067 论文字数:10844,页数:27
摘 要：本文以苯甲醛和壳聚糖为原料通过席夫碱反应并还原合成N-苄基壳聚糖衍生物，用傅立叶变红外光谱对N-苄基壳聚糖进行了红外表征。通过糠醛比色法测定衍生物的吸光度，发现N-苄基壳聚糖衍生物的胆酸结合能力比未经化学修饰的壳聚糖要高。利用Feton试剂产生羟基自由基，采用水杨酸显色法测定N-苄基壳聚糖衍生物的羟基自由基OH·清除能力，结果显示其有一定的清除能力。利用邻苯三酚自氧化法测定N-苄基壳聚糖衍生物对超氧自由基O2-·的清除能力，结果显示也具有一定清除能力，同时探讨了N-苄基壳聚糖衍生物的溶解性及浓度对清除能力的影响。N-苄基取代改性使浸湿效果和溶解性有了一定的改善，使清除自由基能力提高。但减少了NH2，会使清除自由基能力下降，因此N-苄基壳聚糖衍生物清除自由基能力不高。
关键词：壳聚糖；N-苄基壳聚糖衍生物；胆酸结合能力；自由基清除能力
Study on the Preparation and Application of N-benzyl Chitosan Derivatives
Abstract: This paper introduce the synthesis of N-benzyl derivative through benzaldehyde and chitosan Schiff reaction and was characterized by IR. Determination of furfural derivatives by absorbance, we found N-benzyl derivatives of bile acid binding capacity of chitosan was higher than chitosan. Though using Feton reagent of hydroxyl radical and the color determination of salicylic acid, we tested N-benzyl chitosan derivatives of the hydroxyl radical OH-scavenging ability, the results show that it is have scavenging ability. Use of pyrogallol autoxidation method, we tested N-benzyl chitosan derivatives on O2-superoxide free radical scavenging, the results show also has scavenging, and we discussed the N-benzyl chitosan derivatives solubility and concentration on the scavenging effect. The preparation of N-benzyl modified improved solubility , thus enhancing the free radical scavenging. However, a decrease of NH2, the free radical scavenging ability of thus N-benzyl chitosan derivatives was lower.
Key words: Chitosan; N-benzyl chitosan derivatives; Bile acid binding capacity; Free radical scavenging capacity
目 录
中文摘要 I
英文摘要 II
目 录 III
1 绪论 1
1.1 壳聚糖简介 1
1.2 壳聚糖及衍生物的应用 1
1.4 壳聚糖及其衍生物改性方法及性能 3
1.5 壳聚糖的N-苄基化反应（席夫碱反应） 4
1.6 N-苄基壳聚糖衍生物表征 4
1.7 N-苄基壳聚糖衍生物的生物活性 5
1.8 实验思路 6
2 实验部分 8
2.1 材料试剂与设备 8
2.2 实验步骤 8
2.3 结果与讨论 12
2.4 结论与展望 17
致 谢 19
参考文献 20
