【编者按】：网学网理学论文为您提供对乙酰氨基酚的含量测定及有关物质检查参考，解决您在对乙酰氨基酚的含量测定及有关物质检查学习中工作中的难题，参考学习。
论文编号:ZY009 论文字数:7563,页数:21
摘要：本论文建立对乙酰氨基酚含量测定的方法。采用高效液相色谱法和紫外分光光度法。高效液相色谱法以C18为色谱柱，以甲醇-水 (体积比70∶30)为流动相，流速为1.0 mL／min，检测波长为250 nm。对乙酰氨基酚在5～30 μg／mL浓度范围内呈现良好的线性关系(r=0.9997)，回收率为99.6％（RSD=1.9％)。在该色谱条件下对对乙酰氨基酚的杂质对氨基酚进行检查，对氨基酚线性范围0.1 μg/mL ～1.2 μg/mL。紫外分光光度法测定对乙酰氨基酚含量，结果表明，对乙酰氨基酚在2～10 ug／mL范围内线性关系良好，平均回收率为99.9％(RSD=1.1％)。我们建立的两种含量方法操作简便，灵敏度高，重复性好，测定结果准确，可用于对乙酰氨基酚的含量测定。
关键词： 对乙酰氨基酚；对氨基酚；高效液相色谱法；紫外分光光度法
Abstract : The purpose of this article was to establish methods for content determination of acetaminophen. We used High-performance liquid chromatography and ultraviolet spectrophotometry. Chromatographic conditions were C18 column and methanol-water (volume ratio 70:30) as the mobile phase. The flow rate was 1.0 mL / min and the detection wavelength was 250nm. In the range of 5～30 μg／mL, acetaminophen’s concentration showed a good linear relationship (r = 0.9997). The recovery ratio was 99.6% (RSD = 1.9%). The p-aminophenol was detected under the same conditions and its linear range was 0.1μg/mL~1.2μg/mL. UV spectrophotometry was used to determine the content of acetaminophen. In the range of 1~10ug/mL, the concentration showed a good linear relationship and the average recovery ratio was 99.9%(RSD = 1.1%). The two methods were simple, sensitive, reproducible and accurate, which can be used in the content determination of acetaminophen.
Key words： Acetaminophen；p-Aminophenol ；HPLC； UV spectrophotometry
目 录
1. 绪论 ………………………………………………………… 1
1.1 对乙酰氨基酚………………………………………… 1
1.2 选题背景与意义……………………………………… 1
1.3 含量测定方法………………………………………… 2
2. 实验部分 …………………………………………………… 4
2.1 高效液相色谱法……………………………………… 4
2.2 紫外分光光度法……………………………………… 6
3. 结果与讨论 ………………………………………………… 7
3.1 结果…………………………………………………… 7
3.2 讨论…………………………………………………… 12
4. 总结与展望 ………………………………………………… 13
致谢 ……………………………………………………………… 14
参考文献 ………………………………………………………… 15
