响应面法提取车前子多糖的工艺研究

论文编号:ZY055    论文字数:9786,页数:25

摘要：为了确定车前子中多糖热水浸提提取的最佳工艺条件，以多糖得率为指标，对车前子料液比、提取时间、提取温度、提取次数等因素进行了考察，结果表明车前子多糖的较优提取工艺为料液比为1：20、提取时间为3h、提取温度为80℃、提取次数2次。在此基础上采用响应面法对主要工艺参数进行优化并得到回归模型。方差分析得到的优化的工艺参数为：提取时间3.45h、提取次数2.52、提取温度79.91℃、料液比1:20。此工艺条件下提取车前子多糖的得率为（以葡萄糖计）8. 03%。该优化工艺提高了多糖的提取率，取得了较好的成效。
关键词：车前子；多糖；提取工艺；响应面法

Abstract: The optimization experiment of active polysaccharide extraction of Plantago was carried out and the optimal extraction condition was determined, and accordingly to be used in extracting of Plantago polysaccharide. In the experiment Plantago polysaccharide yield was taken as an index, extraction temperature, extraction time, material-water mass ratio and extraction times. The results showed that the better extraction time was 3h, extraction temperature was 80℃, material-water ratio was 1:20, extraction times was 2. In this paper, the conditions of extracting yam polysaccharides were optimized based on response surface method (RSM). Experimental factors and their levels were determined by one-factor tests. Subsequently, the CCD design with 4 factors and 5 levels was performed, and the factors influencing the technological were estimated by quadratic regression analysis with interaction terms. Response surface and contour were finally graphed with the extraction rate as the response value. The results showed that the optimal conditions of polysaccharides extraction were obtained as following, extraction temperature 79.91℃,extraction time 3.45h, the material-water ratio 1:20, extraction times was 3 and that extraction rate could be up to 8.03%.
Keywords: Plantago; polysaccharide; Extraction process; Response surface method

目录
摘要 I
Abstract II
1绪论 1
1.1 车前子简介 1
1.2 多糖提取方法 2
1.3 响应面分析法进行优化 4
1.4 研究的意义和目的 4
2．实验部分 5
2.1 材料与仪器 5
2.2试验方法 5
3 结果与分析 9
3.1 单因素结果 9
3.2 响应面法优化车前子多糖提取工艺 11
3.3 结论 16
4.总结与展望 17
4.1实验总结 17
4.2 展望 17
致谢 19
参考文献 20