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论文编号:SP021 论文字数:7870,页数:17
摘 要
将从土壤中分离出来的8株木霉菌(Trichodema spp.),与节瓜枯萎病原菌进行对峙培养,筛选出拮抗节瓜枯萎病菌镰刀菌的优良木霉菌T04-004菌株,该菌株对节瓜枯萎病菌的抑制率达90%以上。对生防菌木霉菌T04-004进行生物学特性测定,结果表明,在Richard培养液中,T04-004菌株孢子萌发需要一定的营养,其中以PD培养液最好,并且在PDA培养基上菌丝生长最好,产孢量最大。T04-004菌株孢子萌发,菌丝生长和产孢的温度范围为15℃~40℃;T04-004菌株孢子萌发,菌丝生长和产孢的pH值范围为2~10;光照对菌丝的生长影响不显著,但对产孢量的影响较大。
关键词:木霉菌 节瓜枯萎病原菌 拮抗作用 生物学特性
Biological control Effect of Trichodema on Fusarium oxysporum f.sp.benincasae
Abstract: 8 Trichodema were collated from the soil, carries on confronts the Fusarium oxysporum f.sp.benineasae, screens the fine Trichodema T04-004 that oppresses Fusarium oxysporum f.sp.benineasae, This strain to the Fusarium oxysporum f.sp.benineasae ''''s suppression rate achieved above 90%. The biological characterizations of Trichoderma viride T04-004 were screened and identified. The results illustrated that the spore germination of T04-004 strain needs certain nutrition, and the PD nutrient fluid is the best. The strain T04-004 grows well in the PDA culture medium, including the hypha and spore production. The temperature range of spore sprouts, hypha growth and spore production is 15℃~40℃, and the range of pH-value is 2~10. The illumination is not remarkable influence to hypha growth, but significantly affect on the spore production.
Key words: Trichodema; Fusarium oxysporum f.sp.benincasae; antagonism; biological characteristic
目 录
1 前言 1
2 材料与方法 2
2.1 木霉菌的分离纯化 2
2.1.1 木霉菌的分离 2
2.1.2 木霉菌的纯化和保存 2
2.2 拮抗镰刀菌的木霉菌筛选 2
2.2.1 供试菌株和材料 2
2.2.2 对峙培养 2
2.3 木霉菌生物学测定 3
2.3.1 菌种来源 3
2.3.2 环境因子对分生孢子萌发的影响 3
2.3.3 环境因子对菌丝生长和产孢的影响 3
3 结果及分析 4
3.1 木霉菌的分离纯化 4
3.2 拮抗镰刀菌的木霉菌筛选 4
3.3 木霉菌生物学测定 6
3.3.1 环境因子对孢子萌发的影响 6
3.3.2 环境因子对菌丝生长和产孢量的影响 7
4 结论与讨论 10
4.1 木霉菌的分离纯化 10
4.2 拮抗镰刀菌的木霉菌筛选 10
4.3 木霉菌生物学测定 10
4.3.1 关于环境因子对孢子萌发的影响 10
4.3.2 关于环境因子对木霉菌生长速度和产孢量的影响 11
参 考 文 献 12
英 文 摘 要 13
致 谢 14