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论文编号:SP008 字数:5689.页数:11
摘要
用紫外分光光度计对食品中山梨酸含量的测定,实验先测定山梨酸的最大单吸收峰,后用乙醚萃取种类食品中的山梨酸,再在选定的条件下进行测定。测定结果表明,所测样品均合格。最后对实验采用的条件及食品中的其它添加剂造成的影响进行讨论,并且列举了对各类食品相对应的改进方法。采用的方法简便,容易操作。
关键词:紫外分光光度计 山梨酸 无水乙醚 食品添加剂 苯甲酸
Abstract
Determined the content of sorbic acid in food with UV spectrophotometer. Firstly ,determined the sorbic acid’s largest single absorption peak, then the sorbic acid was extracted with ether. The results show that all the samples are qualified. Then discussing the conditions and other additive’s the impact. The improved method for the serious food’s determination was list. The method is simple, easy to operate.
Keywords:UV spectrophotometer Sorbic acid Anhydrous ether Food additive Benzoic Acid
目录
摘要.............................................................................. - 1 -
引言 ............................................................................. - 2 -
1 实验原理........................................................................ - 3 -
2 仪器与试剂...................................................................... - 3 -
2.1 主要仪器...................................................................... - 3 -
2.2 药品 ..........................................................................- 3 -
2.3 食品 ..........................................................................- 3 -
3 实验部分.........................................................................- 3 -
3.1 山梨酸最大波长及标准曲线的测定.................................................- 3 -
3.1.1 标准溶液的配制 ..............................................................- 4 -
3.1.2 最大吸收波长的确定.......................................................... - 4 -
3.1.3 标准溶液吸光度的测定........................................................ - 4 -
3.2 样品处理 ..................................................................... - 4 -
3.2.1 液体样品.....................................................................- 4 -
3.2.2 固体样品.....................................................................- 5 -
3.2.3 样品中山梨酸含量的测定 ......................................................- 5 -
3.2.4 数据处理.....................................................................- 5 -
4 方法与评价.......................................................................- 6 -
4.1 方法的选择.....................................................................- 6 -
4.2 萃取溶剂的选择.................................................................- 6 -
4.3 酸性条件.........................................................................8
4.4 萃取的次数...................................................................... 8
4.5 干扰因素.........................................................................8
5 结果讨论.........................................................................- 8 -
结束语 ........................................................................... - 9 -
致谢 ............................................................................. - 9 -
参考文献 ..........................................................................- 9 -