液相色谱串联质谱法测水产品中的硝基呋喃代谢物

论文编号:SP105  论文字数:6170,页数:18

摘要：由于硝基呋喃类药物对人类健康的潜在危害，欧盟已经禁止其在动物食品生产中使用。采用固相萃取(SPE)前处理净化技术, 高效液相色谱-串联质谱(LC-MS/MS)法测定水产中呋喃它酮(furaltadon)、呋喃西林(furacilin)、呋喃妥因(titrofurantoine)、呋喃唑酮(furazolidonum)代谢产物的残留量。分析物采用多反应监测方法(MRM)，同位素内标法定量。

关键词：硝基呋喃代谢物；水产品；固相萃取；高效液相色谱-串联质谱
  
Highly effective liquid chromatography - series mass spectrography determination pesticide residues nitryl fu nan kind of antibiotic metabolite
Abstract：The use of nitrofurans in food-producing animals has been banned in EU, because they may represent a public health risk. A method has been developed to determine the pesticide residues of furaltadon, furacilin, titrofurantoine and furazolidonum in Aquatic products by LC-MS/MS coupled with the solid phase extraction(SPE).Identification was achieved by using multiple reaction monitoring(MRM). The quantification was performed with isotope as the internal standard.

Key word：nitrofuran metablolites；pesticide residues；SPE；LC-MS/MS
 中文摘要……………………………………………………………………………..1
英文摘要……………………………………………………………………………..1
1     绪论……………………………………………………………………………2
 1.1 前言……………………………………………………………………...2
2    试验部分……………………………………………………………………….4
 2.1试剂和材料………………………………………….……………..… ..4
 2.2仪器和设备………………………………………………………………. 6
 2.3仪器参数与测定   ………………………………………………………  6
3    实验方法……………………………………………………………………….7
      3.1试样制备………………………………………………………………….7
 3.2分析步骤…………………………………………………………………7
 3.3净化步骤 ………………………………………………………………..8
 3.4校正曲线………………………………………………………………….8
结果与分析…………………………………………………………..……….8
4.1过柱量选择………………………………………………………………..8
4.2衍生温度和时间的选择……………………….………………………..10
4.3提取净化方法的选择…………………………….……………………..10
硝基呋喃代谢物衍生物的工作曲线及相关系数…………………………..11
注意事项……………………………………………………………………..14
回收率和精密度……………………………………………………………..14
参考文献……………………………………………………………………..16
致谢…………………………………………………………………………..17

附录A：标准品色谱图