磁性纳米固定化淀粉酶研究

论文编号:ZY027    论文字数:11063,页数:23

摘 要：酶作为一种生物催化剂,因其具有高选择性、催化反应条件温和、无污染等特点,广泛应用于食品加工、医药和精细化工等行业，随着人类对环保的日益关注,酶的应用会更受关注。但游离酶的稳定性差、易失活、不能重复使用,纯化困难等缺点，使其难以在工业中更为广泛的应用。在此条件下,固定化酶的概念和技术得以提出和发展,此外，如何利用天然高分子载体,或利用超临界技术、纳米技术、膜技术等来固定酶,也成为酶工程研究的热点。
 本论文主要研究磁性纳米固定化淀粉酶，它以磁性纳米粒子为载体，制备磁性纳米固定化淀粉酶。然后测定固定化酶的固载率，游离酶和固定化酶的活性,从而确定固定化酶的部分性质。
关键词：固定化；磁性纳米；淀粉酶；活性

Abstract: Enzyme, as a biological catalyst, is widely used in food processing, pharmaceuticals and fine chemical industries for its high selectivity, mild reaction, non-pollution and other good characteristics. As people pay more attention to the environmental protection, the application of enzyme will be more concerned. However, there are many shortcomings of free enzyme, such as poor stability, easy deactivation, non-reuse, difficultly purification and so on. It is more difficult to be widely used in the field of industry. Under these conditions, the concepts and techniques of immobilization of enzyme are mentioned and developed. In addition, how to make use of the natural polymer carrier or to make use of  some advanced technology, such as supercritical technology, nanometer technology, membrane technology ,to immobilize enzyme have become interesting topics on enzymatic engineering.
 This article is mainly study the technology of immobilization of amylase on magnetic nanoparticles. Amylase was immobilization on magnetic nanoparticles. And then try to exam the immobilization yield of the immobilized enzyme,and the enzyme activities of immobilized enzyme and free enzyme to demonstrate some characters of immobilized enzyme.
Keywords：immobilization ; magnetic nanoparticles; amylase; activity

目  录
中文摘要 I
英文摘要 II
目录 III
1. 绪论 1
 1.1  酶的固定化 ...1
 1.2   磁性纳米固定化酶技术 4
 1.3   本文研究内容 5
2. 实验部分 6
 2.1  一般实验条件 6
 2.2  磁性纳米微粒固定化淀粉酶 7
 2.2.3  操作步骤 7
 2.3  Bradford法测定淀粉酶的蛋白质含量 9
 2.4  淀粉酶的活性测定 10
3. 结果与讨论 12
    3.1  淀粉酶的固载率 12
 3.2   固定化酶和游离态酶水解效果的差异 13
 3.3  固定化酶贮存稳定性测定 14
4．总结与展望 16
致谢 18
参考文献 19