【网学提醒】：本文主要为网上学习者提供人造血干细胞扩增的研究进展，希望对需要人造血干细胞扩增的研究进展网友有所帮助，学习一下吧!
资料包括： 论文(5页4432字)
说明：
关键词： 干细胞,人造血
progress on Study of Techniques for Expansion of Human Hematopoietic Stem Cells
造血干细胞（llematopoietic stem cells,HSC）移植可能使遗传血液病、免疫缺陷病及恶性肿瘤等完全治愈，由于HSC移植存在以下不足而受限制：⑴为获得足够量移植用HSC，必须进行大规模的骨髓抽吸或餐周血分离；⑵获得的HSC量有限；⑶HSC输注后产生的成熟细胞的生物动力欠佳，移植后1-3周内并无直接治疗疗效。移植前HSC培养扩增，可解决这些难题。当前主要有两种：⑴细胞因子支持下筛选CD34+细胞的体外扩增；⑵基质支持下的灌注培养。下面就HSC培养的发展、HSC扩增方法以及展望等作一综述。
1 早期HSC培养及启示
1半固体培养体系 1966年，Bradley等介绍了一种新的半固体培养方法（即集落形成法）用以培养骨髓。此法利用胶体凝胶作支托，没有细胞微环境，只能维持造血祖细胞在1-2周内形成细胞集落，不能支持HSC的生长或分化。即使没法加入营养物、生长因子等，培养时间也只能延长到2-4周。由半固体培养体系的期限性可推断，早期原始HSC不能在这样的培养体系中存活、增殖。
目录：
1 早期HSC培养及启示
2 目前HSC的体外扩增方法
3 展望
参考文献：
Bradley TR,et al.The growth of mouse marrow cells in vitro.Aust J ExpBiol Med Sci,1966:44:287
Dexter TM,et al.Conditions controlling the proliferation of haemopoietic stem cells in vitroJ Cells Pysiol,1977;91:335
Gartner S,et al.Long term culture of human bone marrow cells.Proc Natl Acad Sic USA,1980;77:4656.
Sutherland HJ,et al.Alternative mechanisms with and without steel factor support primitive human hematopoiesis,Blood,1993;81:1465
Lansdorp PM,et al.Ontogeny-related change:in proliferative potential of human hematopoietic cells.J Exp Med,1993;178:787
Metcalf D,Hematopoietic regulator:redundancy or subtlety?Blood,1993:82:3515
Heimfied S,et al.Ex vivo expansion analysis from CD34+ HLA-BRI,CD34± and CD38+subest Eep Hematol,1994(Abstr):22:756
Bjomson BH.et al.Inhition of CFU-NM and CFU-Eos by matrure granulocytes,Blood,1984:63:376
Tavassoli M,Hematopoetic survival factro.Exp Hematol,1993;21:1411
Moore MA,Expansion of myeloid stem cells in culture.Semin Hematol,199:32:183
Haylock ND,et al.Ex vivo ecpansion and maturation of peripheral blood CD34+ cells into the myeloid lineage ,Blood,1992;80:4405
Sato Net al.vitro expansion of human peripheral blood CD34+cells,Blood,1993;82:3600
Brugger W,et al.Ex vivo ecpansion of enriched perpheral blood CD34+ progenitor cells by stem cell factor,interleukin-1 beta(IL-1 beta),IL-6,IL-1,interferon-gamma,and erythropoietin Blood,1993:81:2579
Mayani H,et al.Cytokine-induced selectiver expansion and maturation of erythroid versus myeloid cord blood precurrsor cell.Blood,1993;81:3252
Nakahata T,Expansion of hematopoietic progenitors.In:Bajus JL,et al.eds,Education Program of The 26th Congress of The Intemational Society of Haematology Singapore,1996;29-302
Osyka T et al.The effects of interleukin-6 and interleukin-3 on early hematopoietic events in long-term cultures of human marrow,Exp Hematol,1991;19:1042
Koller MR,et al.Long-term culture initiating cell expansion is dependent on frequent medium exchange combined with stromal and other accessory cell effects .Blood,1995;86:1784
Koller MR,et al.Expansion of primitaive human hematopoietic progenitors in a perfusion bioreactor syystem with IL-3,IL-6 and stem cell factor.Biotechnology,1993;11:358
Han CS,et al.In vitro expansion of umbilcal cord blood committed and primitive progenitors.Exp Hematol,1994(Astl):22:723
Verfaillie CM,Direct contact between human primitive hematopoieitc progenitors and bone marrow stuoma is not requied for long-term in vitro hematopoiesis,Blood,1992;79:2821
Guba SC,et al.Bone marrow stromal cells secrete IL-6 and GM-CSF in the absence of inflammatory stimuli:demonstration by serum-fred bioassay:ELSA,and reverse transcriptase polymerase chair,reaction,Blood,1992;80:1190
Schwartz RM,et al.Rapid medium perfusion rate significantly increases the productivity and logevity of human bone marrow cultures.Proc Natl Acad Sci USA,1991;88:6760
Palsson MA,et al.Expansion of CD34+-enriched human bone marrow cells:effct of heeding schedule schedule cytokines and stuoma.Exp Hematol,1994(Abstr):22:726
Koller RM,et al.Reduced oxygen temsion increases hematopoiesis in long-term culture of human stem and progenitor cells from cord blood and bone marrow.Exp Hematol,1992:20:264
Palsson BO,et al.Expansion of human bone marrow progenitor cells in a high cell desity continuous perfusion system.Bio-Technol,1993;11:368
Davis TA,et al.Large scale expansion of human CD34+ progentor cells on porcine endothelial cells in and artifical capillary system,Exp,Hematol,1994(Abstr):22:803
