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【摘要】 目的 观察体外培养的人类角膜缘干细胞对同种异体外周血淋巴细胞的作用。方法 通过植片技术分离人角膜上皮细胞以及角膜缘干细胞。在两种细胞单层上，观察同种异体外周血淋巴细胞对丝裂原刀豆蛋白Ａ（ConA）的增殖反应。另外，将两种细胞培养上清直接转移到由ConＡ刺激的淋巴细胞增殖系统，以观察两种细胞释放某些因子的抑制效应。结果 单层角膜缘干细胞对ConＡ刺激的外周血淋巴细胞增殖反应可以施加有效的抑制效应。角膜缘干细胞培养上清对ConＡ刺激的外周血淋巴细胞增殖系统，在72h分离上清显示有明显抑制效应。结论 角膜缘干细胞通过细胞交互作用和（或）释放某些抑制性因子对淋巴细胞的活化和增殖发挥潜在的抑制作用。
【关键词】 角膜上皮细胞,角膜缘干细胞,细胞培养
Studies on the keratolimbal stem cell to the pericirculation lymphocyte activation and proliferation
【Abstract】 Objective To investigate the regulation of host lymphocyte activation by the allograft of limbal stem cells.Methods Limbal stem cells and the corneal epithelial cells were cultured and isolated from human corneas by explant technique.The mediums of each cells were gathered time and were freeze at different time in -20℃ after purification.Lymphocytes of the other body were cultured in mediums stimulated by Con A.Limbal stem cells or the corneal epithelial cells of the human were co-cultured with the lymphocytes from the other bodys blood.Lymphocyte proliferation assay was assessed by MTT colorimetric determination.Results Significant proliferation of the lymphocytes was shown after adding the Con A.The gathered supernatant of the 72h either from the limbal stem cells or the corneal epithelial cells was shown a potent inhibitory effect of the lymphocytes proliferation.The monolayer domestic rabbits limbal stem cells,showed a significant suppressive effect on the allograft lymphocytes proliferation after mixed lymphocyte culture for 48h,but this phenomenon couldnt be seen in the monolayer corneal epithelium in vitro.Conclusion The results show that there would be a lot of possible mechanisms that worked independently or worked in concert to inhibit the lymphocytes proliferation,but mainly by cell-cell interaction and/or some other soluble factors,and limbal stem cells would take a role on raising the eye immunity.
【Key words】 corneal epithelium; limbal stem cell; cell culture
目录：
1 材料与方法
2 结果
3 讨论
参考文献：
1 洪荣照,吴护平,王印其,等.角膜缘移植联合穿透性角膜移植的临床观察.眼科,1998,7(4):224.
2 龚向明,刘红山,钟兴武,等.角膜缘上皮移植联合角膜移植治疗眼化学烧伤.中国实用眼科杂志,1998,16(8):472.
3 邓宏伟，李辰，陈建苏,等.角膜缘干细胞对外周血淋巴细胞活化和增殖作用的实验研究.眼科研究,2003，21（3）：261-263.
4 Mosmann T.Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.J Immunological Methods,1983,65:55.
5 Coligan JE,Kruisbeek AM,Margululies DH,et al.Current protocols in immunology.New York: Greene Publish in Gand Wiley Inter Science,1991,3-12.
6 Li DQ,Tseng SCG.Three patterns of cytokine expression potentially involved in epithelial-fibroblast interactions of human ocular surface.J Cell Physiol,1995,163: 61.
7 Holland EJ,Djalilian AR,Schwartz GS.Management of aniridic keratopathy with keratolimbal allograft: a limbal stem cell transplantation technique.Ophthalmology,2003,110(1):125-130.
8 Solomon A,Ellies P,Anderson DF,et al.Long-term outcome of keratolimbal allograft with or without penetrating keratoplasty for total limbal stem cell deficiency.Ophthalmology,2002,109(6):1159-1166.
9 Dua HS,Azuara-Blanco A.Limbal stem cells of the corneal epithelium.Surv of Ophthalmol,2000,44(5): 415.
