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【摘要】 目的 观察急性心肌梗死（AMI）患者外周血中CD34-阳性的单个核细胞（CD34-positive mononuclear cells，MNCCD34+）的动员及其变化规律。方法 观察AMI患者外周血中不同时间段MNCCD34+的数量，测量粒细胞集落刺激因子(granulocyte colony-stimulating factor，G-CSF)等5项细胞因子在血浆中不同时间段的变化水平。结果 （1）MNCCD34+从AMI后便开始增加，第7天达到峰值，然后逐渐呈下降趋势，但在第28天MNCCD34+的数目仍然高于第1天。（2）心梗组和对照组MNCCD34+数目在第7天时差异有非常显著性(P＜0.001)。（3）外周血中的细胞因子G-CSF显著增高，且于第7天达到峰值。（4）单因素回归分析显示，循环中MNCCD34+的数目同血浆中G-CSF的水平密切相关(r＝0.379，P＜0.01)。结论 AMI患者外周血中MNCCD34+的数目和G-CSF水平是增高的，且二者密切相关。证明AMI后机体存在骨髓动员和自我修复现象。G-CSF是外周血干细胞的动员剂。
【关键词】 急性心肌梗死,干细胞,流式细胞术,粒细胞集落刺激因子,动员
Endogenic mobilization of peripheral blood stem cells in patients with acute myocardial infarction
【Abstract】 Objective To investigate the mobilization of CD34-positive mononuclear cells(MNCCD34+) in the peripheral blood of the patients with acute myocardial infarction(AMI). Method To examine the quantity of the MNCCD34+ after AMI，and the plasma level of granulocyte colony-stimulating factor(G-CSF) at different times. Results （1）MNCCD34+ increased after the onset of AMI and peaked on day 7，then gradually decreased，but the number on day 28 was still greater than that on day 1. （2） The MNCCD34+ counts were significantly greater in the AMI group than in the control group on day 7 (P＜0.001).（3）G-CSF levels significantly increased and peaked on day 7. （4）Simple regression analysis revealed that the number of circulating MNCCD34+ positively correlated with the plasma levels of G-CSF.(r=0.379，P＜0.01).Conclusion The quantity of MNCCD34+ and G-CSF in the peripheral blood of patients with AMI is higher than the healthy people. Maybe G-CSF correlate with the mobilization.
【Key words】 acute myocardial infarction；stem cell；flow-cytomery；granulocyte colony-stimulating factor；mobilization
目录：
1 对象与方法
2 结果
3 讨论
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